Addgene

We narrowed to 23,361 results for: Nov;

Showing: 6511 - 6540 of 23361 results

Results per page:

Sort results by:

FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AP-1 mu1-GFP

Plasmid

#173432

Purpose

Expression of AP-1 Mu1 subunit with a terminal GFP

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP1M1 (Ap1m1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-L

Plasmid

#182283

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG/ATP11A-HA

Plasmid

#209218

Purpose

Mammalian expression of ATP11A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP11A (ATP11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG/ATP10A-HA

Plasmid

#209215

Purpose

Mammalian expression of ATP10A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10A (ATP10A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG/ATP8B2-HA

Plasmid

#209210

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B2 (ATP8B2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP786_SpyCatcher-EZH2-FL-CO

Plasmid

#211779

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-KRAB

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_cAMPFIRE-L-R279E

Plasmid

#182282

Purpose

Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-N-T205I

Plasmid

#177954

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (T205I) (N )

Use

Tags

Expression

Mammalian

Mutation

T205I

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Article

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Use

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG/ATP11C-HA

Plasmid

#209220

Purpose

Mammalian expression of ATP11C

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP11C (ATP11C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-INPP4B WT

Plasmid

#161995

Purpose

Mammalian expression vector containing INPP4B. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP4B (INPP4B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Tags

Expression

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-GRAM1b

Plasmid

#211494

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

Depositor

Yasunori Saheki

Article

Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTone-gata2aECE-Kaede

Plasmid

#132976

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

Kaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge