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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 4341 - 4360 of 18824 results

  4. pSmart-EF1a-Nla-cas5

    Plasmid
    #178878
    Purpose
    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas5c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178878-plasmid-map-sequence-id-351751

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  5. pSmart-EF1a-Nla-cas7

    Plasmid
    #178879
    Purpose
    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas7c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178879-plasmid-map-sequence-id-351739

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  6. pSmart-EF1a-Nla-cas8

    Plasmid
    #178880
    Purpose
    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas8c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178880-plasmid-map-sequence-id-351750

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  7. pSmart-EF1a-Nla-cas11

    Plasmid
    #178882
    Purpose
    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas11c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178882-plasmid-map-sequence-id-351753

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  8. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  9. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  11. mKikGR-PCNA-19-SV40NLS-4

    Plasmid
    #57328
    Purpose
    Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mKikGR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. SpCas9-ABE8e(V106W)

    Plasmid
    #198547
    Purpose
    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198547-plasmid-map-sequence-id-388882

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  14. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  16. mPA-GFP-PCNA-19-SV40NLS-4

    Plasmid
    #57154
    Purpose
    Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. RPL25NLS-GFP-PRA (102)

    Plasmid
    #24041
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP and PRA
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Available since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only
Showing: 4341 - 4360 of 18824 results