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pGBW-m4046832

Plasmid

#145691

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046907

Plasmid

#149262

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;Myc

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046953

Plasmid

#145764

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF7b

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pGBW-m4046891

Plasmid

#145749

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4136454

Plasmid

#149529

Purpose

Bacterial expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 13, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-5A, 6A mMCL1-OM

Plasmid

#45818

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

5A, 6A mMCL-1-OM

Use

Tags

Expression

Mammalian

Mutation

Changed Arginine to Alanine at 5th and 6th amino …

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pGBW-m4046919

Plasmid

#145615

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-TGF beta type I receptor (mL45)

Plasmid

#34701

Depositor

Ying Zhang

Article

Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

N265A, D267A, N268A

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pGBW-m4046890

Plasmid

#145740

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4095420

Plasmid

#149523

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

d1-745 d1063-1945

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046949

Plasmid

#145604

Purpose

Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac-His-Flag-Tipin

Plasmid

#22890

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag- and His

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGBW-m4046735

Plasmid

#149234

Purpose

Bacterial expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046883

Plasmid

#145693

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046841

Plasmid

#145578

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge