We narrowed to 22,489 results for: SCR

1. pEBB-GFP-hBEN L38&45P

   Plasmid

   #22183

   Depositor

   Ananda Roy

   Article

   Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

   Insert

   hBEN (GTF2IRD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Luecine 38 to Proline and Leucine 45 to P…

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. GFP-ZMYND8

   Plasmid

   #65401

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   ZMYND8 (ZMYND8 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PBRM1

   Plasmid

   #65387

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

   Plasmid

   #194279

   Purpose

   Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized VP64 dSaCas9 VP64 T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. proE-cad178-Luc-mEbox

   Plasmid

   #42082

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   E-cadherin (CDH1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   contains E-cadherin promoter region from -178 to …

   Promoter

   E-cadherin

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-tomato SOX2-sgRNA

   Plasmid

   #196190

   Purpose

   Editing human SOX2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-ZMYND11

   Plasmid

   #65402

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   ZMYND11 (ZMYND11 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Sox2-t2A-GFP

    Plasmid

    #127537

    Purpose

    Expresses human SOX2 and eGFP via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PHIP

    Plasmid

    #65389

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PHIP (PHIP Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

    Plasmid

    #184742

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMax_mPlum_C207G/T208C

    Plasmid

    #177831

    Purpose

    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum_C207G/T208C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C207G + T208C

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CA-Stat3-t2A-mCherry

    Plasmid

    #127540

    Purpose

    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders …

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX2-HA-GATA3 FL

    Plasmid

    #89835

    Purpose

    Express GATA3 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Foxf1 HA pShuttle

    Plasmid

    #122120

    Purpose

    Expression of Foxf1 in mammalian cells

    Depositor

    Paul Herring

    Article

    Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

    Insert

    Foxf1 (Foxf1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sox2-t2A-mCherry

    Plasmid

    #127538

    Purpose

    Expresses human SOX2 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    MLV promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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