We narrowed to 4,711 results for: lum

1. pUCmini-RELA-PGK-tdTomato

   Plasmid

   #228054

   Purpose

   Homologous donor template for RELA knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-RELC-PGK-tdTomato

   Plasmid

   #228056

   Purpose

   Homologous donor template for RELC knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Clinical Immunology Communications, Volu

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PRLSS-yATOM_SH2-KDEL

   Plasmid

   #209714

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-yATOM_SH2-KDEL
   

   Tags

   PRLSS

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

   Plasmid

   #209718

   Purpose

   Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-NbGFP_miRFP670nano3-KDEL
   

   Tags

   PRLSS

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PRLSS-Clover-KDEL

   Plasmid

   #209720

   Purpose

   Mammalian expression of clover localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-Clover-KDEL
   

   Tags

   PRLSS

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGWB302-er-mCherry

   Plasmid

   #186287

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Expression

   Bacterial

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQIq_MRGS_HIS8_(27B3)_FLAG

   Plasmid

   #199613

   Purpose

   Encodes DARPin-FLAG 27B3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27B3
   

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_ E3_5 _hFc

   Plasmid

   #199617

   Purpose

   Encodes DARPin-hFc E3_5 for mammalian expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-hFc E3_5 (control)
   

   Tags

   hFc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK2-mMOV10-3'UTR

   Plasmid

   #178905

   Purpose

   dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   3'UTR mMov10 (Mov10 Mouse)
   

   Use

   Luciferase

   Promoter

   SV40 promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAPV-FLAG-DsRed2-ER-5

    Plasmid

    #170620

    Purpose

    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-FLAG-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPV-FLAG-DsRed2-ER-5

    Plasmid

    #170621

    Purpose

    Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    EPV-FLAG-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAPV-mEmerald-DsRed2-ER-5

    Plasmid

    #170681

    Purpose

    Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-mEmerald-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAPV-DsRed2-mEmerald-ER-5

    Plasmid

    #170715

    Purpose

    Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-mEmerald-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEE22

    Plasmid

    #161787

    Purpose

    Hi-SC2 reporter delivery plasmid

    Depositor

    François-Xavier Barre

    Article

    Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

    Insert

    Hi-SC2 reporter
    

    Use

    Conjugative plasmid to deliver hi-sc2 reporter

    Mutation

    Himar site included MmeI site, tandem loxP sites,…

    Promoter

    n/a

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JGI_018307

    Plasmid

    #158831

    Purpose

    Rare earth element alcohol dehydrogenases

    Depositor

    Joint Genome Institute

    Article

    Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

    Insert

    Rhodovulum
    

    Use

    Synthetic Biology

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQP-2363

    Plasmid

    #138975

    Purpose

    Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
    

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD-MTS2 (12)

    Plasmid

    #101698

    Purpose

    MTS2 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mPapaya1-CytERM-N-17

    Plasmid

    #56352

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    CytERM
    

    Tags

    mPapaya1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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