We narrowed to 995 results for: KO

1. pcDNA3-HA PTEN

   Plasmid

   #78776

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG PTEN

   Plasmid

   #78777

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EWB-DIO-cyan pre-eGRASP(p30)

   Plasmid

   #111583

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p30)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

   Plasmid

   #111584

   Purpose

   An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrmScarlet-I-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3

   Plasmid

   #48743

   Purpose

   Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   truncated mPer2 promoter/enhancer region (-1128 to +1060)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   truncated promoter region (see pGL6)

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

7. CMV10-3xFlag Skp2 WT

   Plasmid

   #81115

   Purpose

   mammalian expression of Skp2 WT

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

   Plasmid

   #111590

   Purpose

   An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrTagRFP-T-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV PGK1-3xHA

   Plasmid

   #220913

   Purpose

   Expresses PGK1-3xHA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-3xHA (PGK1 Human)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Tetoffbidir -Alb-luc

    Plasmid

    #164078

    Purpose

    AAV vector with bidirectional promoter controls expression firefly Luciferase with TRE and Tet-off transactivator

    Depositor

    Shu Uin Gan

    Article

    Gan et al J Gene Med. 2019 Jan;21(1):e3067. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Albumin

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBR_attB(C31)_FRT

    Plasmid

    #183761

    Purpose

    phiC31-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSFDuet1-His-TEV-OTC_Ub

    Plasmid

    #172134

    Purpose

    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

    Depositor

    Shang-Te Danny Hsu

    Article

    Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

    Insert

    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti hSyn PGK1-HALO

    Plasmid

    #220910

    Purpose

    Neuronal specific expression of the fusion construct PGK1-HALO

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-HALO (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-ASAP2f

    Plasmid

    #183757

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL2

    Plasmid

    #48742

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +386)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-4T-1-Skp2 WT

    Plasmid

    #81117

    Purpose

    bacterial expression plasmid of Skp2

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pNEXdelta-EGFP-ApAF

    Plasmid

    #13335

    Depositor

    Bong-Kiun Kaang

    Article

    Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

    Insert

    Aplysia activating factor
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGL4

    Plasmid

    #48744

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1536 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +1536)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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