We narrowed to 22,433 results for: TIM;

1. mSTIM1_1-250-CFP

   Plasmid

   #69552

   Purpose

   Expresses a STIM1_1-250 fragment mammalian cells

   Depositor

   Yubin Zhou

   Article

   Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

   Insert

   mSTIM1_1-250
   

   Use

   Tags

   Cyan Fluorescent Protein (CFP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONRPEX18Tp-SceI-pheS

   Plasmid

   #111608

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCdrA-gfp(ASV)C

   Plasmid

   #111615

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniTn7(Gm)PA1/04/03-eyfp-a

   Plasmid

   #111620

   Purpose

   eYFP Fluorescent tagging of bacteria at their Tn7-site

   Depositor

   Tim Tolker-Nielsen

   Article

   Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

   Insert

   PA1/04/03-eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBAD-yhjH

   Plasmid

   #111613

   Purpose

   Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

   Depositor

   Tim Tolker-Nielsen

   Article

   Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

   Insert

   yhjH
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A52V

   Promoter

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniTn7(Gm)PA1/04/03-ecfp-a

    Plasmid

    #111619

    Purpose

    eCFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-ecfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC37

    Plasmid

    #111618

    Purpose

    Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

    Depositor

    Tim Tolker-Nielsen

    Article

    Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

    Insert

    PpqsA-gfp(ASV)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONRPEX18Gm-SceI-pheS

    Plasmid

    #111609

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid

    #193942

    Purpose

    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

    Depositor

    Greg Delgoffe

    Article

    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

    Insert

    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

    Plasmid

    #231602

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag_codon_optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-TIMD4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156731

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TIMD4 (TIMD4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

    Plasmid

    #169930

    Purpose

    Overexpression of 1-270AA CycK-GFP fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Insert

    CCNK (CCNK Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW-codon optimized catalytically inactive PHGDH

    Plasmid

    #154903

    Purpose

    Expresses codon optimized catalytically inactive PHGDH in mammalian cells

    Depositor

    Michael Pacold

    Article

    Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Three mutations: D175N, R236K, H283A

    Promoter

    TRE

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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