Addgene

We narrowed to 22,876 results for: TIM;

Showing: 421 - 440 of 22876 results

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pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Latimeria chalumnae

Plasmid

#75356

Purpose

Nt His-tag Latimeria chalumnae Sacsin 94-1368

Depositor

Kalle Gehring

Insert

Sacsin Latimeria chalumnae 94-1368

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmAgo1_572-984-optim_K

Plasmid

#146728

Purpose

Bacterial Expression of DmAgo1_572-984-optim

Depositor

Elisa Izaurralde

Insert

DmAgo1_572-984-optim (AGO1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-SIRPT1-Latimeria chalumnae

Plasmid

#75324

Purpose

GST fusion Latimeria chalumnae Sacsin 94-1368

Depositor

Kalle Gehring

Insert

Sacsin Latimeria chalumnae 94-1368

Use

Tags

GST-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Use

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV,&Rhamnose

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Lck-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129627

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Lck-cpmTq2-Calcium-lifetime-sensor

Use

Tags

Lck

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Use

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
mNeongreen-pGBD(PKN1 codon optimized)

Plasmid

#129634

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding doamin of PKN1(30 - 100) codon optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pETM-60-HsAGO2-448-859-optim_K

Plasmid

#146729

Purpose

Bacterial Expression of HsAGO2_448-859-optim

Depositor

Elisa Izaurralde

Insert

HsAGO2_448-859-optim (AGO2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA29 (yeast codon optimized)

Plasmid

#188441

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA29 (yeast codon optimized)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-732pUC19-tNGFR-P2A-STIM1

Plasmid

#186119

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STIM1 (STIM1 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 (optimized for N-terminal knock-in)

Plasmid

#172608

Purpose

Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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