Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,139 results for: 112

Showing: 4381 - 4400 of 13139 results

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SIN40C.EFS.eGFP.mArid3a-3'UTR-WT

Plasmid

#169299

Purpose

Constitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3'UTR-WT (Arid3a Mouse)

Use

Lentiviral

Promoter

EFS

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.EFS.eGFP.ARID3A-3'UTR-WT

Plasmid

#169297

Purpose

Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A-3'UTR-WT (ARID3A Human)

Use

Lentiviral

Promoter

EFS

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5AR280H

Plasmid

#166957

Purpose

Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A-R280H (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A-R280H

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA + S580A POLI

Plasmid

#131241

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert Pol iota se…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ADCK5 gRNA (BRDN0001162224)

Plasmid

#77317

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK5 (ADCK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK5 gRNA (BRDN0001144876)

Plasmid

#77318

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK5 (ADCK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK5 gRNA (BRDN0001145727)

Plasmid

#77319

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK5 (ADCK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK5 gRNA (BRDN0001162211)

Plasmid

#77320

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK5 (ADCK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTKD1 gRNA (BRDN0001147356)

Plasmid

#77126

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTKD1 (FASTKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTKD1 gRNA (BRDN0001146718)

Plasmid

#77127

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTKD1 (FASTKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTKD1 gRNA (BRDN0001144859)

Plasmid

#77125

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTKD1 (FASTKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GAK gRNA (BRDN0001146263)

Plasmid

#76471

Purpose

3rd generation lentiviral gRNA plasmid targeting human GAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GAK (GAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GAK gRNA (BRDN0001148048)

Plasmid

#76472

Purpose

3rd generation lentiviral gRNA plasmid targeting human GAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GAK (GAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146337)

Plasmid

#75707

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146285)

Plasmid

#75708

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001147842)

Plasmid

#75709

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001144911)

Plasmid

#75706

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pZ M2rtTA_CAGG TetON-Sox10

Plasmid

#115240

Purpose

RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

SOX10 (SOX10 Human)

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' AHDC1

Plasmid

#236672

Purpose

5' AAVLINK plasmid for AHDC1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

AHDC1 (AHDC1 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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