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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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p15a-AsCas12f-blaZ

Plasmid

#171609

Purpose

AsCas12f1-based E. coli genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Expression

Bacterial

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPP537

Plasmid

#158803

Purpose

pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-SP1

Plasmid

#232649

Purpose

expresses SP1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP1 (SP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gs)

Plasmid

#230011

Purpose

Expresses anti-mCherry PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dxs

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT10

Plasmid

#223382

Purpose

T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpRY-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Article

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-anti-CD3/anti-CD19 BiTE

Plasmid

#230005

Purpose

Gal4-dependent expression of secreted BiTE

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-anti-CD3/anti-CD19 BiTE

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

Plasmid

#169097

Purpose

Cas9 and Citrine expression in transfected cells

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

Cas9-2A-Citrine

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Epac2

Plasmid

#118314

Purpose

Expression of mouse Epac2

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

Insert

Epac2 (Rapgef4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

Plasmid

#171438

Purpose

Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-c-Jun

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230016

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

Lentiviral

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gs)

Plasmid

#230017

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dxs

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLX208-FLiCRE2-TF

Plasmid

#234523

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

CD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TL024_lenti_hSyn:HaloTag-GluA1

Plasmid

#192517

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(G12)

Plasmid

#230013

Purpose

Expresses anti-mCherry PAGER(G12) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dx12

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230010

Purpose

Expresses anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQAT01

Plasmid

#223373

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

AtUBQ10-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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