We narrowed to 15,368 results for: NOL

1. pGL2B -1538/+64

   Plasmid

   #24942

   Depositor

   Stephen Smale

   Article

   Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

   Insert

   IL-10 promoter (Il10 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2ST

   Plasmid

   #59384

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-dfrA
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAMD1-2

   Plasmid

   #61564

   Purpose

   Standardized mini-Tn5 delivery plasmid, kanamycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-mE1

   Plasmid

   #32534

   Depositor

   Jorge Eduardo Azevedo

   Article

   Carvalho et al Mol Biotechnol. 2011 Oct 20.

   Insert

   E1, ubiquitin-activating enzyme (Uba1 Mouse)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-TREX1

   Plasmid

   #27219

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-HisD-miniGFP2

   Plasmid

   #188897

   Purpose

   Express 6xHis-Tag miniGFP2 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV-U6+27-Tornado-RhoBAST

    Plasmid

    #196341

    Purpose

    Overexpression of circular RhoBAST

    Depositor

    Murat Sunbul

    Article

    Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

    Insert

    Tornado-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Vimentin-7

    Plasmid

    #55156

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG1237 FIREmate-ECFP-Fis1

    Plasmid

    #205173

    Purpose

    Expresses FIREmate-ECFP-Fis1 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Fis1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV_Cas9_puro

    Plasmid

    #65655

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    5 ' FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19 - T7 pro - IRES - EGFP

    Plasmid

    #138586

    Purpose

    Express EGFP with an upstream T7 promoter in pUC19 backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_SpG‐ABE8e(V106W)

    Plasmid

    #187069

    Purpose

    ABE8e(V106W)-SpG

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpG‐ABE8e(V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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