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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Stargazin-EBFP2-FRB

Plasmid

#222417

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

Cacng2 (Cacng2 Mouse)

Tags

EBFP2-HA-FRB

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Di-iPGM

Plasmid

#180241

Purpose

Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Dirofilaria immitis iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-LAP2beta

Plasmid

#222416

Purpose

To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LAP2beta (Tmpo Rat)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-BAF

Plasmid

#222414

Purpose

To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

BAF (BANF1 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

Plasmid

#212809

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

Plasmid

#212804

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-P2A-3xFLAG-Ubc7

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

Plasmid

#212803

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-VSV

Plasmid

#212743

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

Plasmid

#212800

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Mutation

HOIP(C885A)

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

Plasmid

#212814

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo(K63R)

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-mCherry-FLAG

Plasmid

#212798

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Tags

mCherry-FLAG

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His7-NUbo-GFP

Plasmid

#212791

Purpose

Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His7-NUbo-GFP

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo-GFP

Plasmid

#212792

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo-GFP

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-E3(63) I227A-VSV

Plasmid

#212757

Purpose

Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63) I227A

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Pib1(I227A)

Promoter

CUP1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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