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pKlab639-HBEGF-2a-mRuby3-WPRE

Plasmid

#249030

Purpose

This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mRuby3

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab672-HBEGF-2a-mTagBFP2-WPRE

Plasmid

#249031

Purpose

This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mTagBFP2

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab821-pcDNA3.1-HALO-3xFLAG

Plasmid

#249007

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

HALO

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab965-PX459-BRCA1-Nterm-guide1

Plasmid

#249005

Purpose

This plasmid expresses the Cas9/gRNA that will target the HBEGF gene locus

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

Cas9

Use

CRISPR

Tags

3xFLAG

Promoter

chicken beta-actin promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1580_ECOR31HamAB_CoPurification

Plasmid

#223371

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1444_ECOR31HamA_purification

Plasmid

#223369

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamA

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamA

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1445_ECOR31HamB_purification

Plasmid

#223370

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamB

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamB

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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ttLbCas12a-intron

Plasmid

#241901

Purpose

ttLbCas12a (LbCas12a-D156R)-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

ttLbCas12a (LbCas12a-D156R)-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-RRV-intron

Plasmid

#241902

Purpose

LbCas12a-RRV-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

LbCas12a-RRV-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pYSGDLuc

Plasmid

#236297

Purpose

Dual luciferase reporter for measuring recoding efficiencies in yeast

Depositor

Pasha Baranov

Article

Fenton et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8553_ pEML41003-tufBpst4-15

Plasmid

#237866

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufB

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufB

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8359_pA3A-CBECas9-ALSpro197

Plasmid

#234353

Purpose

Base editor targets to ALS Pro197 locus

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

ALS

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pColE1-MP-Violacein

Plasmid

#240205

Purpose

Violacein biosynthesis pathway induced by CRISPRa

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

vioA-vioB-vioC-vioD-vioE

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSC-pGAL10-CRISPRa

Plasmid

#240210

Purpose

Yeast vector for integration of pGAL10-dCas9 and pGAL10-MCP-VPR at LEU2 locus

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

MCP-VPR
dCas9

Expression

Yeast

Promoter

pGAL10

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MRscRNA

Plasmid

#240214

Purpose

Mammalian vector for transient expression of theophylline-responsive MR-scRNA with mCherry transfection marker

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TET3G promoter

Expression

Mammalian

Promoter

U6

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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