We narrowed to 279,909 results for: ins

1. pcDNA3.1+HIF1a-AS1_1200_unspliced

   Plasmid

   #194176

   Purpose

   Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   HIF1a-AS1 (HIF1A-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-MPP8-delta55-118-10xHis

   Plasmid

   #194180

   Purpose

   Expresses MPP8-delta55-118_10xHis

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-delta55-118 (chromo domain deletion)

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-MPP8-delta2-441-10xHis

   Plasmid

   #194181

   Purpose

   Expresses MPP8-delta2-441_10xHis

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-delta2-441

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-MPP8-delta442-860-10xHis

   Plasmid

   #194182

   Purpose

   Expresses MPP8-delta442-860_10xHis

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-delta442-860

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-MPP8-delta600-728-10xHis

   Plasmid

   #194183

   Purpose

   Expresses MPP8-delta600-728_10xHis

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-delta600-728 (4xANK deletion)

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HisGB1-CITED(216-242)+CBPTAZ1(340-439)

   Plasmid

   #189961

   Purpose

   Coexpression of CBP TAZ1 and CITED in E coli

   Depositor

   Peter Wright

   Article

   Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Inserts

   CITED2 (216-242) (CITED2 Human)
   CBP TAZ1 (340-439) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

   Plasmid

   #189963

   Purpose

   Coexpression of CBP TAZ1 and CITED2 in E coli

   Depositor

   Peter Wright

   Article

   Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Inserts

   CITED2 (216-269) (CITED2 Human)
   CBP TAZ1 (340-439) (Crebbp Mouse)
   

   Expression

   Bacterial

   Mutation

   L243A, E245A, L246A

   Promoter

   t7

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

   Plasmid

   #189962

   Purpose

   Expression of CBP TAZ1 and CITED2 in E coli

   Depositor

   Peter Wright

   Article

   Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Inserts

   CITED2 (216-246) (CITED2 Human)
   CBP TAZ1 (340-439) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

   Plasmid

   #185701

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(FCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid

    #185705

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PDCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

    Plasmid

    #185703

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(SCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

    Plasmid

    #185704

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

    Plasmid

    #185702

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(BCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKHH030_sgAMBRA1#2

    Plasmid

    #174147

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKHH030_sgAMBRA1#1

    Plasmid

    #174146

    Purpose

    Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid

    #114076

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    S542R and K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSEss-chimeric-IgM-mouse/human_M18

    Plasmid

    #91738

    Purpose

    Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Residues 203-239 (EU numbering) were exchanged to…

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUSEss-CHIg-mM-Asp212Ser_M18

    Plasmid

    #91739

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asp212 changed to Ser (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_ABCB9_p.R281L

    Plasmid

    #82840

    Purpose

    Gateway Donor vector containing ABCB9, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ABCB9 (ABCB9 Human)
    

    Use

    Gateway entry vector

    Mutation

    R281RL; 582_590delISLVSQEPVinsVCARAWATL; 592_595d…

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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