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pWUKI Cas1 Cas2 G74A

Plasmid

#80101

Purpose

Encodes Cas1 and Cas2 G74A

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

G74A

Availability

Academic Institutions and Nonprofits only

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pCambia1300-PacI

Plasmid

#44183

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

Plasmid

#43803

Purpose

Encodes a gRNA that targets Can1.Y in yeast.

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

CAN1.y gRNA (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SMC3

Plasmid

#222554

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SMC3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMC3 (SMC3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV h c-MYC IRES GFP

Plasmid

#18119

Depositor

John Cleveland

Insert

c-MYC (MYC Human)

Use

Retroviral

Tags

GFP

Mutation

The CUG which is the start site for Myc I is pres…

Available Since

June 9, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-GFP-WPRE

Plasmid

#211488

Purpose

expresses GFP under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-GFP-WPRE

Plasmid

#211490

Purpose

expresses GFP under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GFP-WPRE

Plasmid

#211489

Purpose

expresses GFP under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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reporter-gT1

Plasmid

#47320

Purpose

TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymScarletI3_T2A_mTurquoise2

Plasmid

#219829

Purpose

equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymScarletI3_T2A_mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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JD630

Plasmid

#160394

Purpose

Vitis GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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JD689

Plasmid

#160396

Purpose

citrus GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

citrus GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PAX6a

Plasmid

#222576

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PAX6a

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PAX6a (PAX6 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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