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TLCV2-sgHPDL #9

Plasmid

#174166

Purpose

knock out HPDL in human cell lines

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

sgRNA against HPDL

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pD2-dcas9-J23101-GFP

Plasmid

#122260

Purpose

Constitutive dCas9 and GFP expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

dCas9

Use

Synthetic Biology

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB105-mTalin

Plasmid

#100584

Purpose

Binary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Citrine-mTalin (Tln1 Mouse)

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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POPFOP

Plasmid

#34849

Depositor

Paul Sternberg

Article

Green et al Cell. 2008 Aug 22;134(4):646-56.

Insert

Mutated TCF binding sites

Tags

let-858 3'UTR and mCherry

Expression

Bacterial

Promoter

pes-10 minimal promoter

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET156P CFAP36-3C-muGFP-10His (Sus scrofa)

Plasmid

#238214

Purpose

Bacterial expression vector of porcine CFAP36 with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 (CFAP36 )

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG1C-eIF3ac

Plasmid

#240039

Purpose

Human eIF3a and eIF3c subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3a, c

Tags

eIF3a: TEV_3xFLAG and eIF3c: 3C_STREP II

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLSUBR

Plasmid

#245489

Purpose

Binary Plasmid for plant transformation expressing RUBY from the Lactuca sativa polyubiquitin 4 gene promoter and the terminator from the same gene. Kanamycin plant selection

Depositor

Beth Rowan

Insert

pLsUBI-RUBY-tLSUBI

Expression

Plant

Promoter

Polyubiquitin 4 gene (LOC111919935) from lettuce

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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NylB_pET29b

Plasmid

#215414

Purpose

Expression construct for NylB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB

Expression

Bacterial

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Tt-NylC_pET28a

Plasmid

#215431

Purpose

Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Tt-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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INT_Cargo_T7_CbFDH

Plasmid

#212140

Purpose

Cargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Article

Fung et al Biomaterials. 2022 Aug;287:121661. doi:

Insert

formate dehydrogenase

Expression

Bacterial

Promoter

PT7

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207847

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P

Plasmid

#207846

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE1_Z

Plasmid

#148115

Purpose

Mammalian Expression of HshnRNPE1

Depositor

Elisa Izaurralde

Insert

HshnRNPE1 (PCBP1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE2iso4_Z

Plasmid

#148119

Purpose

Mammalian Expression of HshnRNPE2iso4

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso4 (PCBP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPE2iso4_Z

Plasmid

#148141

Purpose

Mammalian Expression of HshnRNPE2iso4

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso4 (PCBP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC4

Plasmid

#183199

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC4

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+-dCas9-olEzh2

Plasmid

#180216

Purpose

Ezh2 fused to dCas9 for H3K27me3 epigenome editing

Depositor

Hiroyuki Takeda

Article

Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

Insert

enhancer of zeste 2 polycomb repressive complex 2 subunit

Tags

FLAG and dCas9

Expression

Bacterial

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-eGFP

Plasmid

#136098

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_eGFP

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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