We narrowed to 6,822 results for: pcdna3.1

Showing: 4501 - 4520 of 6822 results

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hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Ga13_2-mTq2

Plasmid

#112933

Purpose

Human Galpha13 tagged with mTurquoise, inserted at residue 128

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

Galpha13

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP2-Mb2Tomato-2A (SO240)

Plasmid

#99614

Purpose

To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4495

Plasmid

#80338

Purpose

mammalian expression MbCpf1 nuclease and Mb crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
MbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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PA-RL-BAD

Plasmid

#78859

Purpose

DULIP positive control (bait) for assay establishment

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

BCL2 associated agonist of cell death (BAD Human)

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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iBlueberry T2A mCherry

Plasmid

#72434

Purpose

Expressing of iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

iBlueberry T2A mCherry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tpr-Si

Plasmid

#60918

Purpose

Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

TPR (TPR Human)

Expression

Mammalian

Mutation

Silent point mutations in siRNA target region; E1…

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP mCherry

Plasmid

#45235

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, mCherry

Tags

full length CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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H2A-Bbd-GFP

Plasmid

#45173

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al J Cell Biol. 2001 Jan 22;152(2):375-84.

Insert

H2AFB1 (H2AFB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
PCx35-pcDNA

Plasmid

#42920

Depositor

John O'Brien

Article

O'Brien et al J Neurosci. 2004 Jun 16;24(24):5632-42.

Insert

Connexin 35

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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YFP-Gamma 5

Plasmid

#36044

Depositor

Narasimhan Gautam

Article

Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

Insert

G protein subunit Gamma 5 (Gng5 Rat)

Tags

His and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
LRRK2-K1906M

Plasmid

#35946

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDKIER

Plasmid

#37093

Depositor

David Wang

Insert

KI polyomavirus early region (KPV_gp4, KPV_gp5 KI polyomavirus)

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 8

Plasmid

#36106

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 8 (GNG8 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only