We narrowed to 5,339 results for: pcdna3.1

1. pCDNA3-SREBP-1 (A317-318; A341)

   Plasmid

   #204194

   Purpose

   Mammalian expression of SREBP-1 (Isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. InsRA-eGFP

   Plasmid

   #79795

   Purpose

   Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Tags

   eGFP extracellular

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEmax_2star_IVT

   Plasmid

   #227674

   Purpose

   plasmid for PEmax** mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   PEmax_2_star
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS0035

   Plasmid

   #131125

   Purpose

   Mammalian Target-AID expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. RF-118-UB-BRCA1

   Plasmid

   #78960

   Purpose

   Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

   Depositor

   Richard Fahlman

   Article

   Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   3x Flag and ubiquitin

   Expression

   Mammalian

   Mutation

   delta 1-1155

   Promoter

   CMV

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MC4R

   Plasmid

   #233630

   Purpose

   encodes the human melanocortin 4 receptor, without any tags

   Depositor

   Christian Gruber

   Article

   Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

   Insert

   MC4R (MC4R Human)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Luc-PS9

   Plasmid

   #177942

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaMK2rep-S603A

    Plasmid

    #239623

    Purpose

    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Article

    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

    Insert

    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Tags

    Spot, myc, and sGFP2

    Expression

    Mammalian

    Mutation

    changed Serine 603 to Alanine

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SacI ML GFP Strand 11 Long

    Plasmid

    #164120

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Long
    

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SacI ML GFP Strand 11 Short

    Plasmid

    #164121

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Short
    

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Opto-GPR149

    Plasmid

    #106049

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR149 (GPR149 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hACE2

    Plasmid

    #1786

    Purpose

    Mammalian expression of human ACE2

    Depositor

    Hyeryun Choe

    Article

    Li et al Nature. 2003 Nov 27. 426(6965):450-4.

    Has Service

    Cloning Grade DNA

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Luc-T20

    Plasmid

    #177941

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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