We narrowed to 25,531 results for: pET

1. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a SznF E281A

   Plasmid

   #139591

   Purpose

   expresses SznF E281A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E281A
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 281 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a SznF H225A

   Plasmid

   #139590

   Purpose

   expresses SznF H225A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H225A
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed histidine 225 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SznF E215A

   Plasmid

   #139565

   Purpose

   expresses SznF E215A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E215A
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-DDX43-NT-G46D

   Plasmid

   #134588

   Purpose

   Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G154D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-DDX43-FL-G84D

   Plasmid

   #134577

   Purpose

   Express human DDX43 mutant (full-length with G84D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-G84D (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G84D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET14b-dabAB2 D353A

   Plasmid

   #133016

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14b-dabAB2 C351A

   Plasmid

   #133014

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-GreBdm-D41N

   Plasmid

   #129691

   Purpose

   express E. coli GreB E82C/C68S/D41N

   Depositor

   Jeff Gelles

   Article

   Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GreB E82C C68S D41N (greB E. coli)
   

   Tags

   his6

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJUMP18-RBS-pET_R

    Plasmid

    #127013

    Purpose

    Basic Part R- RBS; pET vector Ribosome Binding Site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part RBS-pET_R
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK-petD 3'UTR

    Plasmid

    #123571

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    petD 3'UTR
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-SOMA-T679A

    Plasmid

    #118936

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21d-colEdes7/Imdes7

    Plasmid

    #107115

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes7/Imdes7
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21d-colEdes8/Imdes8

    Plasmid

    #107116

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes8/Imdes8
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21d-colEdes9/Imdes9

    Plasmid

    #107117

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes9/Imdes9
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21d-colEdes10/Imdes10

    Plasmid

    #107118

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes10/Imdes10
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21d-colEdes11/Imdes11

    Plasmid

    #107119

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes11/Imdes11
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge