We narrowed to 15,228 results for: NOL

1. LbCas12a-nuCPE

   Plasmid

   #213748

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-LbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBait

   Plasmid

   #165146

   Purpose

   Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroX-Tight-MCS_PGK-GpNLuc

   Plasmid

   #70185

   Purpose

   Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK198 (GAL-OsTIR1)

   Plasmid

   #140655

   Purpose

   GAL-OsTIR1(WT)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT9R

   Plasmid

   #98305

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB519

   Plasmid

   #74861

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB511

   Plasmid

   #74853

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF0688 Kp03_attP-mCherry donor

    Plasmid

    #193467

    Purpose

    Promoterless mcherry donor for Kp03

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT950

    Plasmid

    #228290

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMM0646

    Plasmid

    #129007

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_836

    Plasmid

    #216088

    Purpose

    Cas9 [Sp] knockout targeting CD55, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD55 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZ_P-GAP-eGFP

    Plasmid

    #126717

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. eNme2-C TadCBEd

    Plasmid

    #193841

    Purpose

    Express TadCBEd (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG sHRPb-FKBP-post mGRASP

    Plasmid

    #73146

    Purpose

    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-FKBP-post mGRASP
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xHA-miniTurbo_pAS32

    Plasmid

    #118222

    Purpose

    expresses 3xHA-tagged miniTurbo in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. C3 luc (T1 del)

    Plasmid

    #11358

    Depositor

    Donald McDonnell

    Article

    Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

    Insert

    C3 promoter (C3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    none

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-CaMKIIa-ChRger2-TS-EYFP

    Plasmid

    #127238

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDUAL_tacI

    Plasmid

    #155170

    Purpose

    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAF0451 Si74_attP-mCherry donor

    Plasmid

    #193470

    Purpose

    Promoterless mcherry donor for Si74

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB516

    Plasmid

    #74858

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGWB417

    Plasmid

    #74811

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.2/Capsid

    Plasmid

    #120816

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Capsid ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. attB-puro-GA donor

    Plasmid

    #182138

    Purpose

    attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEL031

    Plasmid

    #137900

    Purpose

    STU2.0 SpyCas9 tRNA system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmIL-6 mut NF-kB

    Plasmid

    #61293

    Purpose

    drives luciferase from mouse IL-6 promoter with mutation in NF-kB site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated NF-kB binding site from GTGGGATTTTCCCA to…

    Promoter

    IL-6 promoter with mutant NF-kB binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only