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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTW087

Plasmid

#64756

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW087

Plasmid

#64756

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector RO1_1

Plasmid

#158010

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_1

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_2

Plasmid

#158011

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_2

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_2

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO1_5

Plasmid

#158012

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_5

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_5

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO1_8

Plasmid

#158013

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_8

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO1_9

Plasmid

#158014

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_9

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_1

Plasmid

#158015

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_1

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_5

Plasmid

#158016

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_5

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_5

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_6

Plasmid

#158017

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_6

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_6

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_9

Plasmid

#158018

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_9

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_10

Plasmid

#158019

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_10

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_15

Plasmid

#158020

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_15

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_15

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_20

Plasmid

#158021

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_20

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_20

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge