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pTHSSd_14

Plasmid

#59952

Purpose

Triple split (at 67, 601-SZ)

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Three-fragment T7* RNA polymerase

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-invitro(KaganD86)

Plasmid

#52012

Purpose

Used for in vitro expression of the human MAVS CDS

Depositor

Jonathan Kagan

Publication

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSIBR2.0-818-Cas12a-NT

Plasmid

#235168

Purpose

Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

Depositor

Constantinos Patinios

Publication

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

SIBR2.0-818-Cas12a

Expression

Bacterial

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRel-Hyg

Plasmid

#227582

Purpose

Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

Depositor

Chengzhang Fu

Publication

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLBS-EF-QUE37C

Plasmid

#129338

Purpose

ATP measurement, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMIGR AMPK WT

Plasmid

#27564

Depositor

Morris Birnbaum

Insert

AMP Acticated Protein Kinase (Prkaa2 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pJA55

Plasmid

#59935

Purpose

gRNA for cleavage near sqt-1(e1350) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA2

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pPH37

Plasmid

#35102

Depositor

John McCusker

Publication

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1MX4

Use

Yeast replicating

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTETRIS-cargo

Plasmid

#126033

Purpose

Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

Depositor

Mauro Calabrese

Publication

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Type

Empty backbone

Use

Luciferase; Piggybac

Expression

Mammalian

Promoter

TRE

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMCGL

Plasmid

#29307

Depositor

Nils Walter

Publication

Pereira et al PLoS One. 2010 . 5(9):e12953.

Insert

Glucosamine-6-phosphate activated ribozyme

Expression

Bacterial

Promoter

T7

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 FL (1-440)

Plasmid

#210425

Purpose

Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

Depositor

Carsten Sachse

Publication

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOGG011

Plasmid

#113985

Purpose

Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pBBR1

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Gateway Cloning

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Tags

6xHis

Expression

Bacterial

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQLinkHD-SYNZIP1

Plasmid

#80647

Purpose

Encodes the synthetic coiled coil peptide SYNZIP1

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP1

Tags

6xHis

Expression

Bacterial

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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