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Plasmid#66277PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertF2RL1 (F2RL1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA HCAR3
Plasmid#226811PurposeTet-inducible lentiviral vector for 2xHA HCAR3 expressionDepositorInsertHCAR3 (HCAR3 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_006009.2PromoterAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA HCAR3
Plasmid#226807PurposeGateway entry vector for 2xHA HCAR3DepositorInsertHCAR3 (HCAR3 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_006009.2PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
HCA1-DuET
Plasmid#213307PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCA1 (HCA1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A)
Plasmid#208088PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.DepositorInsertLTB4R(T308A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(S310A)
Plasmid#208089PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.DepositorInsertLTB4R(S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationS310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A,S310A)
Plasmid#208090PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.DepositorInsertLTB4R(T308A,S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308A, S310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R
Plasmid#208091PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationnonePromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDi-P2A-mCherry
Plasmid#204358PurposeAAV vector for miniDi expression under the control of human synapsin promoterDepositorInsertminiDi-P2A-mCherry
UseAAVTagsmCherryExpressionMutationThe third intracellular loop (ICL3) of hM4Di was …PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDq-P2A-mCherry
Plasmid#204357PurposeAAV vector for miniDq expression under the control of human synapsin promoterDepositorInsertminiDq-P2A-mCherry
UseAAVTagsmCherryExpressionMutationThe third intracellular loop (ICL3) of hM3Dq was …PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only