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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 698 results for: Bacillus

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Showing: 441 - 460 of 698 results

  1. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  10. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

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  11. pGEX4-4T-1 BCP1 NTase

    Plasmid
    #161812
    Purpose
    Expresses SMODS/CD-NTase from Bacillus phage Bcp1
    Depositor
    Douglas Feldman
    Insert
    phage BCP1 SMODS/CD-NTase enzyme
    Tags
    His and TEV
    Expression
    Bacterial
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    161812-plasmid-map-sequence-id-319427

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  12. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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  17. pAdeasy-CMV-AE-EF1α-R2

    Plasmid
    #194928
    Purpose
    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis
    Depositor
    Limei Wang
    Article
    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.
    Inserts
    AE
    EEF1A1 (EEF1A1 Human)
    R2
    Use
    Adenoviral
    Tags
    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c
    Mutation
    a 48 bp linker (Gly3ser)4 was added between the t…
    Promoter
    CMV and EF1a
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    194928-plasmid-map-sequence-id-391107

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  20. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid
    #171817
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171817-plasmid-map-sequence-id-338126

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Showing: 441 - 460 of 698 results