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EKAR2G_design1_mTFP_175_Venus_wt

Plasmid

#39828

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_227_Venus_wt

Plasmid

#39833

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_159_Venus_wt

Plasmid

#39823

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pDEST17_A1LigN_EC

Plasmid

#22296

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

A1 domain NAD+ dependent DNA ligase N

Tags

His tag

Expression

Bacterial

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
pDEST-DB

Plasmid

#215482

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pMH180_pDEST_CMV_UBR5_Flag

Plasmid

#247931

Purpose

Overexpression vector for FLAG-tagged UBR5.

Depositor

Benjamin Ebert, Jonathan Tsai

Article

Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1

Insert

UBR5 (UBR5 Human)

Tags

FLAG (DYKDDDDK)

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3

Plasmid

#242154

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV P1.1.3

Expression

Bacterial

Mutation

AraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_R2_LDelK

Plasmid

#242156

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE/RAMPhaGE Linker Library variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV LDelK

Expression

Bacterial

Mutation

AraC: E3K, R146L, A152T, R251L; Left Linker: GS -…

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

Plasmid

#242152

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R2_C1

Plasmid

#242149

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R5_C1

Plasmid

#242150

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_WT

Plasmid

#242148

Purpose

Expression Plasmid containing a LacI repressed sd2 WT AraC cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_WT

Plasmid

#242151

Purpose

Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

Plasmid

#242153

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5 S170 LOV

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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