We narrowed to 15,654 results for: NOL

1. GGE021

   Plasmid

   #120778

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T2-3_Lip2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE094

   Plasmid

   #120787

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Zeta-SfiI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE091

   Plasmid

   #120786

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Zeta-SfiI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p183_LTJ_sgRNACD90.2_A

   Plasmid

   #82671

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD90.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE254

   Plasmid

   #120789

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GGE255

   Plasmid

   #120788

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3_ctrl_CP-candidate_luc+

   Plasmid

   #86392

   Purpose

   Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Type

   Empty backbone

   Use

   Luciferase

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FB367

   Plasmid

   #203630

   Purpose

   Module for the expression of geneticin resistance and Nanoluciferase.

   Depositor

   Jose Marcos

   Article

   Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

   Insert

   PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub
   

   Use

   Synthetic Biology

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-GFP-panda-Caspase-1/4b

   Plasmid

   #183371

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4b
   

   Use

   Retroviral

   Tags

   Myc

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. JME5000

    Plasmid

    #129661

    Purpose

    EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GGE207

    Plasmid

    #120792

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown-Mfe
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p236_LTJ_sgRNAFoxp3sf/J

    Plasmid

    #82676

    Purpose

    sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 sf/J
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p240_LTJ_sgRNAFoxp3K276X

    Plasmid

    #82675

    Purpose

    sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 K276X
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p204_LTJ_sgRNACD45.2_R2

    Plasmid

    #82673

    Purpose

    sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p163_LTJ_sgRNACD90.1

    Plasmid

    #82677

    Purpose

    sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a(+)::NL

    Plasmid

    #141289

    Purpose

    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSTAP-seq_fly-ctrl

    Plasmid

    #86380

    Purpose

    Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Type

    Empty backbone

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3_zfh1_CP-candidate_luc+

    Plasmid

    #86391

    Purpose

    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Luciferase

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge