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Plasmid#236245PurposeCloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoterDepositorInsertmTagBFP2 fused to the plasma membrane targeting sequence CAAX2
UseAAVTagsmTagBFP2ExpressionMutationPromoterU6 and human Synapsin1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-Sec61b WPRE
Plasmid#236228PurposeAAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.DepositorInsertmEmerald-Sec61b (SEC61B Human, Synthetic)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_K262A
Plasmid#225728PurposeTo express Mce3R mutant K262A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationK262APromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelG
Plasmid#225729PurposeTo express MelG (Rv1937) in E. coliDepositorInsertmelG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-VapBC35
Plasmid#225733PurposeTo express VapBC35(Rv1962) Toxin antitoxin in E. coliDepositorInsertvapBC35
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T_Mce3R
Plasmid#225726PurposeTo express Mce3R in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_R53A
Plasmid#225727PurposeTo express Mce3R mutant R53A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationR53APromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-TrxC
Plasmid#225742PurposeTo express TrxC(Rv3914) in E. coliDepositorInsertTrxC
UseTags6xHisExpressionBacterialMutationTrxCPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-TrxB2
Plasmid#225741PurposeTo express TrxB2(Rv3913) in E. coliDepositorInsertTrxB2
UseTags6xHisExpressionBacterialMutationTrxB2PromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelI
Plasmid#225730PurposeTo express MelI (Rv1939) in E. coliDepositorInsertmelI
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelK
Plasmid#225732PurposeTo express MelK (Rv1941) in E. coliDepositorInsertmelK
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelJ
Plasmid#225731PurposeTo express MelJ (Rv1940) in E. coliDepositorInsertmelJ
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS09
Plasmid#67160Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS09
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationL414T within GCaMP6fPromoterT7Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS06
Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
UseTagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-Jph3 WPRE
Plasmid#236238PurposeAAV expression of human Junctophilin3 N-terminally tagged with HaloTagDepositorInsertJunctophilin 3 (JPH3 Human)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mCherry-Jph3 WPRE
Plasmid#236237PurposeAAV expression of human Junctophilin3 N-terminally tagged with mCherryDepositorInsertJunctophilin 3 (JPH3 Human)
UseAAVTagsmCherryExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR FGFR1-CD3ε-FusionRed
Plasmid#188632PurposeHuman FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertFGFR1 (FGFR1 Human, Synthetic)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrFGFR1
Plasmid#183994PurposeOptically controlled eDrFGFR1 expression in mammalian cellsDepositorInserteDrFGFR1 (FGFR1 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG182 FGFR1 3'UTR mut
Plasmid#12009DepositorInsertN15 3'UTR mut (FGFR1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only