We narrowed to 3,069 results for: T2A

1. CA-Stat3-t2A-mCherry

   Plasmid

   #127540

   Purpose

   Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   Stat3 (Stat3 Mouse)
   

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders …

   Promoter

   CMV

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-dCas9-5xPlat2AflD

   Plasmid

   #82560

   Purpose

   Expression vector for Cas9 peptide array (linker length: 22aa).

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD
   

   Expression

   Mammalian

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-GFP-MT2A

   Plasmid

   #11613

   Depositor

   Steven Johnson

   Insert

   metallothionein 2A (MT2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSXneo 270(T2AG3)

   Plasmid

   #12403

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   270(T2AG3)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2C-EGFP-t2a-mCD4

   Plasmid

   #216213

   Purpose

   Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

   Depositor

   Michelle Percharde

   Article

   Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

   Insert

   CD4 (Cd4 Mouse)
   

   Tags

   GFP-T2A

   Expression

   Mammalian

   Mutation

   CD4 transmembrane domain only

   Promoter

   MERVL

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSXneo 135(T2AG3)

   Plasmid

   #12402

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   135(T2AG3)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF3BGX-T2A-lexAp65

   Plasmid

   #138393

   Purpose

   RMCE vector for generating C-terminal T2A-lexA-p65 fusion

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-lexAp65
   

   Expression

   Insect

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-gCarvi-T2A

    Plasmid

    #190333

    Purpose

    Fluorescent reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    gCarvi
    

    Use

    Unspecified

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

    Plasmid

    #32628

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Sce
    

    Use

    Lentiviral

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-rm_gCarvi-T2A

    Plasmid

    #190334

    Purpose

    Ratiometric reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    ratiometric gCarvi
    

    Use

    Unspecified

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

    Plasmid

    #85436

    Purpose

    Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab and anti-CTLA4 scFv
    

    Use

    Lentiviral

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Klf2-t2A-mCherry

    Plasmid

    #127539

    Purpose

    Expresses mouse Klf2 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Klf2 (Klf2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid

    #169333

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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