We narrowed to 2,357 results for: T2A

1. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

   Plasmid

   #160046

   Purpose

   For expressing the full paradoxical circuit

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Inserts

   osTIR1
   iaaH
   iCasp9
   BlastR
   

   Use

   Synthetic Biology

   Tags

   mAID, mCherry, and mGFPmut3

   Expression

   Mammalian

   Mutation

   Promoter

   no promoter, T2A is used and pEF1s

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

   Plasmid

   #199487

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

   Plasmid

   #199488

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

   Plasmid

   #199489

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

   Plasmid

   #199490

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

   Plasmid

   #199491

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

   Plasmid

   #199492

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

   Plasmid

   #173861

   Purpose

   Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

   Plasmid

   #173862

   Purpose

   Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

    Plasmid

    #127560

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    EGFP-D102G see depositor comments below

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

    Plasmid

    #231208

    Purpose

    Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid

    #231205

    Purpose

    Single transcript cageIL-10 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L147I, R148H, I203V, L290V

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

    Plasmid

    #231207

    Purpose

    Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L147I, R148H, I203V, L290V

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113611

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    mu opioid receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

    Plasmid

    #192193

    Purpose

    Lenti-BCMA-CAR-PRODH2

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113610

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    beta 2 adrenergic receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113608

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    PAR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113609

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    PAR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113612

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    S1P1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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