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We narrowed to 10,970 results for: T7

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Showing: 441 - 460 of 10970 results

  1. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

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  2. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

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  3. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

    Enlarge

  4. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  5. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid
    #208972
    Purpose
    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208972-plasmid-map-sequence-id-415827

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  6. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  7. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

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  13. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  14. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  15. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid
    #52133
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and C478S, C631S, C643S,…
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid
    #208290
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpCas9(D10…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208290-plasmid-map-sequence-id-410848

    Enlarge

  20. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

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Showing: 441 - 460 of 10970 results