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Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
HB101 endA::frt
Bacterial Strain#45473DepositorBacterial ResistanceStreptomycinAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
OpLac1
Bacterial Strain#52697PurposeKIlac with a codon optimized lacZ using preferred codon at every positionDepositorBacterial ResistanceNoneAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
HB101 endA::frt lambda pir
Bacterial Strain#45472DepositorBacterial ResistanceStreptomycinAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
∆cosσε ∆HG
Bacterial Strain#197132PurposeDeficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.DepositorBacterial ResistanceHygromycinAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
OpLac2
Bacterial Strain#52699PurposeKIlac with a codon optimized lacZ using codons for a subset of amino acids read by transfer RNAs that are the most highly charged during amino acid starvationDepositorBacterial ResistanceNoneAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 ΔrecBCD
Bacterial Strain#176581PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.DepositorBacterial ResistanceNoneAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 ΔrecB
Bacterial Strain#176580PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.DepositorBacterial ResistanceNoneAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB
Bacterial Strain#176582PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-WT-no_introns
Plasmid#131044PurposeExpresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTRDepositorInsertTPI-Wildtype-no introns (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NG0750 pCI-TPI-PTC48-4H
Plasmid#65803PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-PTC1
Plasmid#131043PurposeExpresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTRDepositorInsertTPI-PTC1 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC1PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-4MS2-xrRNA-4H
Plasmid#108371PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
NG0888 pCI-TPI-PTC48-HBB
Plasmid#65802PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTagsHBB (beta-globin 3'UTR for probe binding)ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only