We narrowed to 5,425 results for: buc

1. pGI3EM31

   Plasmid

   #135491

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mCerulean3
   

   Use

   Tags

   mCerulean3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB367

   Plasmid

   #110277

   Purpose

   gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Insert

   gHEEE_02
   

   Use

   Tags

   10-His, Tev recognition peptide, and mature DsbC …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB367

   Plasmid

   #110277

   Purpose

   gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Insert

   gHEEE_02
   

   Use

   Tags

   10-His, Tev recognition peptide, and mature DsbC …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB812

   Plasmid

   #60160

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   Promoter

   RNR1pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB812

   Plasmid

   #60160

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   Promoter

   RNR1pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre-3G

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre-3G

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

    Plasmid

    #224403

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD02_CBASS_Escherichia_coli_BIDMC103

    Plasmid

    #224402

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD02_CBASS_Escherichia_coli_K71-77

    Plasmid

    #224398

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli K71-77

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Phospholipase_EcCdnE_DisA-like
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge