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pLNHA-C1-HsDCP1b_H

Plasmid

#146505

Purpose

Mammalian Expression of HsDCP1b

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1b (DCP1B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_G

Plasmid

#146401

Purpose

Mammalian Expression of HsDCP2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP2 (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBruno_N

Plasmid

#147028

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-dsRNAres_N

Plasmid

#147029

Purpose

Insect Expression of DmNot1-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmNot1-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBruno_N

Plasmid

#147030

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

Plasmid

#146690

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G and one non silent G56…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

Plasmid

#146691

Purpose

Insect Expression of DmHPat-del57-499-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

Plasmid

#146695

Purpose

Insect Expression of DmHPat_57-968-del500-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-del500-684 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

Plasmid

#146464

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation T477C and one non silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS1

Plasmid

#174301

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1_J

Plasmid

#146688

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Mutation

two non silent mutations Q194R and I1008T compare…

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pOP004

Plasmid

#185985

Purpose

Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

Depositor

Georgi Belogurov

Article

Huang et al Methods Enzymol. 2022;675:207-233. doi:

Insert

Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer

Expression

Bacterial

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsXRN1_1174-1649-Strep_AH

Plasmid

#148901

Purpose

Bacterial Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

Plasmid

#167899

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

Plasmid

#167897

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pOP005

Plasmid

#186030

Purpose

Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

Depositor

Georgi Belogurov

Article

Huang et al Methods Enzymol. 2022;675:207-233. doi:

Insert

Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only