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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-RavZ C258A

Plasmid

#250879

Purpose

Transient mammalian expression of GFP-RavZ C258A

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

RavZ (ravZ Legionella pneumophila)

Expression

Mammalian

Mutation

C258A

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-RavZ

Plasmid

#250878

Purpose

Transient mammalian expression of GFP-RavZ

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

RavZ (ravZ Legionella pneumophila)

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK125

Plasmid

#245147

Purpose

pJAK081 carrying Pcwp2-mScarlet-I

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mScarlet-I

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK201

Plasmid

#245149

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 1 (AAGTCCTCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK202

Plasmid

#245150

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 2 (TCTTGACCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK203

Plasmid

#245151

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 3 (AACAACACC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK204

Plasmid

#245152

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 4 (AACAGGTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK205

Plasmid

#245153

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 5 (ACCGATTAG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK206

Plasmid

#245154

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 6 (CACTTATGC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK207

Plasmid

#245155

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 7 (CCTCCAACT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK208

Plasmid

#245156

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 8 (CGAGGACAT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK209

Plasmid

#245157

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 9 (CTGGTTCTA)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK210

Plasmid

#245158

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 10 (GGATGTTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK211

Plasmid

#245159

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 11 (GTCACCAGT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK212

Plasmid

#245160

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 12 (AGTGTAACG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW003

Plasmid

#245129

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum flgB promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PflgB-bitlucopt

Expression

Bacterial

Promoter

PflgB

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW004

Plasmid

#245130

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum Cspa_RS22190 promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcspa_RS22190 -bitlucopt

Expression

Bacterial

Promoter

Pcspa_RS22190

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge