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pPOTv7-hygro-3HA

Plasmid

#247594

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pNK7190

Plasmid

#219763

Purpose

MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

nnLuz, nnH3H, nnCPH

Expression

Plant

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(EP)-blast-mNG

Plasmid

#221051

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK201

Plasmid

#207131

Purpose

Type 2 ptac promoter part

Depositor

Nicholas Shikuma

Publication

Alker et al mBio. 2023 Aug 2:e0150223. doi: 10.1128/

Insert

ptac promoter

Expression

Bacterial

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM042_P15678_MultiGeneBackbone

Plasmid

#216467

Purpose

Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM041_P15678_SingleGeneBackbone

Plasmid

#216466

Purpose

Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Publication

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA106

Plasmid

#209339

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-moxNG

Plasmid

#190222

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

moxNeonGreen

Use

Unspecified

Promoter

read through

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Esp3I

Plasmid

#197836

Purpose

Level 1 module 5 with Esp3I expansion point

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-Ppyr9eh

Plasmid

#201443

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

Ppyr9eh

Use

Unspecified

Promoter

read through

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:Ppyr9eh

Plasmid

#201441

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG:Ppyr9eh

Use

Unspecified

Promoter

read through

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mScarletI:His

Plasmid

#201098

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mNG:His

Plasmid

#201095

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-tagRFPt

Plasmid

#201085

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-tagRFPt

Plasmid

#201084

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Esp3I

Plasmid

#197832

Purpose

Level 1 module 3 with Esp3I expansion point

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-BsaI

Plasmid

#197820

Purpose

Level 1 module 3 with BsaI expansion point

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-BsaI

Plasmid

#197824

Purpose

Level 1 module 5 with BsaI expansion point

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-1-BsaI

Plasmid

#197816

Purpose

Level 1 module 1 with BsaI expansion point

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge