We narrowed to 43,795 results for: Eng
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pEGFP-N1-zebrafish lyn(C466A)–GFP
Plasmid#35957DepositorInserthumanized zebrafish lyn (lyn Zebrafish)
TagsEGFPExpressionMammalianMutationC466APromotercmvAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-ArchT-GFP
Plasmid#86536PurposeRetrovirus expressing optogenetic silencer ArchT-GFPDepositorInsertArchT
UseRetroviralTagsGFPExpressionMammalianPromoterSynapsin I (human)Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG484
Plasmid#91194PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)DepositorInsertnTaCas9_H840A+gUbi8+donor
UseCRISPRExpressionPlantAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
drd4a_R (TAL 3007)
Plasmid#35999DepositorInsertTAL array targeting drd4a (drd4a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3306
Plasmid#91102PurposeModule C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoterFMV 34SAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D1
Plasmid#61490Purposeluciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2H4
Plasmid#67094PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
tol2-mpx-lyn C466A-GFP
Plasmid#35038DepositorAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-stop
Plasmid#113745PurposeContains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.DepositorInsertstop cassette
UseCRISPR; Gateway entry vectorMutationAdded 3 stop codons to the original sequence; see…Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 MAPK13
Plasmid#67077PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS47 C20orf139 3'UTR mut
Plasmid#14490DepositorInsertC20orf139 3'UTR mutant (SRXN1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENExpressionMammalianPromoterSFFVAvailable SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1010
Plasmid#91030PurposeModule A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoterZmUbiAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1002
Plasmid#91029PurposeModule A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoterAtUbi10Available SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C/T180C
Plasmid#66182PurposeExpresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112C, T180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C/L171C
Plasmid#66178PurposeExpresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105C, L171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_5
Plasmid#91172PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_6
Plasmid#91171PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_D10A (nickase)
UseCRISPRExpressionPlantMutationD10AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_3
Plasmid#91161PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A (nickase)
UseCRISPRExpressionPlantMutationD10AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMOD_A1410
Plasmid#91035PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertTaCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterZmUbiAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC440
Plasmid#91225Purposeprotoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRExpressionPlantAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC441
Plasmid#91218Purposeprotoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRExpressionPlantAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only
