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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_hTLN2-FL-LBD

Plasmid

#164841

Purpose

Expression of GFP tagged FL human talin2 mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-GFP

Expression

Mammalian

Mutation

Lipid binding deficient mutations

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-ccdb

Plasmid

#140864

Purpose

Backbone for making sgRNAs.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Gateway destination vector

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab37

Plasmid

#140886

Purpose

Expression of zebrafish GFP-tagged Rab37 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab37

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826S-sgRNA500

Plasmid

#149618

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0826S

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNA500

Plasmid

#149617

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNA500

Plasmid

#149559

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I54V)

Plasmid

#136608

Purpose

Expresses DANCER-3 mutant (reversion I54V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I54V reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F34A)

Plasmid

#136606

Purpose

Expresses DANCER-3 mutant (reversion F34A)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F34A reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I7L)

Plasmid

#136605

Purpose

Expresses DANCER-3 mutant (reversion I7L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I7L reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F3Y)

Plasmid

#136604

Purpose

Expresses DANCER-3 mutant (reversion F3Y)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F3Y reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP-YTHDF1(D401N)-C

Plasmid

#155354

Purpose

Lentivector to express human YTHDF1(264-559) with D401N mutation and CRY2olig-BFP at the N-terminal

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1(264-559)

Use

Lentiviral

Expression

Mammalian

Mutation

D401N

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNAmreB

Plasmid

#149634

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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