We narrowed to 5,114 results for: mcs

1. XanthoMoClo Xanthobacter Genetic Toolkit

   Plasmid Kit

   #1000000259

   Purpose

   Use this modular cloning kit to assemble plasmids for expression in Xanthobacter and Roseixanthobacter.

   Depositor

   Michael Springer

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

2. pBiFC-HA-hCaspase 2 prodomain-VC155

   Plasmid

   #49261

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pBIFC-flag-hCaspase 2 prodomain-VN173

   Plasmid

   #49262

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pBiFC-HA-hCaspase 2 CARD-VC155

   Plasmid

   #49259

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   Caspase-2 CARD (CASP2 Human)
   

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   deleted aa 123-435

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

   Plasmid

   #49258

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pBiFC-flag-hCaspase 2 CARD-VN173

   Plasmid

   #49260

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 CARD (CASP2 Human)
   

   Tags

   Flag and split Venus N

   Expression

   Mammalian

   Mutation

   delete aa 123-435

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. T7pr-His6-MBP-TEV-dCas9-BirA*

   Plasmid

   #159994

   Purpose

   Bacterial expression of dCas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   dCas9-BirA*
   

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7pr-His6-MBP-TEV-Cas9-BirA*

   Plasmid

   #159993

   Purpose

   Bacterial expression of Cas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   Cas9-BirA*
   

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTetOFF SNCA-A53T

   Plasmid

   #215372

   Purpose

   All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   SNCA (SNCA Human)
   

   Expression

   Mammalian

   Mutation

   Ala53Thr

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HR700_HA2TP53_Ex5

    Plasmid

    #228860

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. HR700_HA2TP53_Ex6

    Plasmid

    #228861

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. HR700_HA2TP53_Ex7

    Plasmid

    #228862

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. HR700_HA2TP53_Ex8

    Plasmid

    #228863

    Purpose

    Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

    Depositor

    Thorsten Stiewe

    Article

    Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAAV.CAG.LSL.tdTomato

    Plasmid

    #100048

    Purpose

    AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdtomato
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV pCAG-FLEX-tdTomato-WPRE

    Plasmid

    #51503

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    pCAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV phSyn1(S)-tdTomato-WPRE

    Plasmid

    #51506

    Purpose

    Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV5

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    phSyn1

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF CAG luc-EGFP-cre puro

    Plasmid

    #67503

    Purpose

    Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    luciferase-EGFP-cre
    

    Use

    Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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