We narrowed to 32,602 results for: Eng

1. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Promoter

   Pgrac

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK_TFAP2a_enh_249_Cerulean

   Plasmid

   #130615

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   TFAP2a_enh_249
   

   Use

   Enhancer reporter

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reporter-1

   Plasmid

   #180218

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK_Axud1_enh_394_Citrine

   Plasmid

   #130625

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Axud1_enh_394
   

   Use

   Enhancer reporter

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tg (mpx:mCherry-2A-Rac2D57N)

   Plasmid

   #58934

   Purpose

   expresses dominant negative rac2 tagged with mcherry in neutrophils

   Depositor

   Anna Huttenlocher

   Article

   Deng et al Dev Cell. 2011 Oct 18;21(4):735-45. doi:

   Insert

   mcherry-2a-Rac2D57N (rac2 Zebrafish)
   

   Tags

   mcherry2a

   Mutation

   changed Aspartate 57 to Asparagine

   Promoter

   mpx

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-cas9-2A-mCherry

   Plasmid

   #107592

   Purpose

   An entry vector with cas9 -2A-mCherry

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   cas9-2A-mCherry
   

   Use

   CRISPR

   Tags

   2A-mCherry

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT11

   Plasmid

   #223383

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT08

   Plasmid

   #223380

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-cMYC

   Plasmid

   #216510

   Purpose

   Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   cMYC
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_Ado1.0mut

    Plasmid

    #153287

    Purpose

    Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Reporter-2

    Plasmid

    #180219

    Purpose

    Surrogate reporter plasmid for the assay of targeted RNA editing

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Insert

    Surrogate reporter
    

    Use

    Lentiviral

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LES2A-CRE

    Plasmid

    #167933

    Purpose

    Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    mScarlet-P2A-CRE
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORF1-2

    Plasmid

    #206024

    Purpose

    An expression vector for mouse LINE1 ORF1-2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1-2 derived from mouse LINE1
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TwinStrep-SUMO-NLS-BhCas12b_v4

    Plasmid

    #158615

    Purpose

    For protein expression of BhCas12b_v4

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    NLS-BhCas12b_v4
    

    Tags

    6xHIS-TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHelper_AraC/pBad-Tnpase (camR)

    Plasmid

    #213909

    Purpose

    Arabinose-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    AraC/pBad ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. gRNA_GFP-T2

    Plasmid

    #41820

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT07

    Plasmid

    #223379

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBES1-LacOx50-blast

    Plasmid

    #210221

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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