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pGEX-6p-1 Sesn3

Plasmid

#61875

Purpose

bacterial expression of a GST-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only
pICH49477

Plasmid

#50324

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, DsRed variant of RFP (Discosoma sp.)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41388

Plasmid

#50253

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBUN276

Plasmid

#60673

Purpose

overexpression of M. tuberculosis ddl gene in Mycobacterium

Depositor

Raul Barletta

Article

Feng et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

ddl

Use

E.coli-mycobacterium shuttle vector

Tags

6 codons of M. bovis BCG hsp60

Promoter

BCG Hsp60

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pICH45173

Plasmid

#50259

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS1B (AT5g38430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH78141

Plasmid

#50293

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (Oryza sativa)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM5331

Plasmid

#50294

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30010

Plasmid

#50305

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, mEOS2 photoactivatable fluorescent protein (Lobophyllia hemprichii)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44157

Plasmid

#50258

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL13002

Plasmid

#50266

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb ) + 5'UTR, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44222

Plasmid

#50287

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR CMV1 (Cucumber Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH44188

Plasmid

#50289

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR BSMV (Barley Stripe Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EKAREN5-c

Plasmid

#246761

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (original YPet - mTurquoise-GL pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-c

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-y

Plasmid

#246762

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (synonymous variant YPet - mTurquoise2 pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-y

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Δpvc10

Plasmid

#243742

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-FLAG_Δpvc10

Plasmid

#243738

Purpose

PVC structural operon - FLAG-tagged baseplate (Pvc12) - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc12-FLAG_ Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-asB539

Plasmid

#127394

Purpose

Expresses amcyan fluorescent gene and sRNA nt 539-559

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Cas13bt1-non-targeting (pJK2)

Plasmid

#229054

Purpose

Cloning backbone for Cas13bt1 crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-Cas13bt1-non-targeting (pEZ25)

Plasmid

#229055

Purpose

Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge