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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM03

Plasmid

#251701

Purpose

Tetracycline resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pJKR-H-benM

Plasmid

#73925

Purpose

Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate biosensing apparatus

Available Since

Jan. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS (L4-R1)

Plasmid

#61374

Purpose

5' Gateway Entry Vector Containing QUAS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QUAS

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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mOrange-N1

Plasmid

#54499

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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mRaspberry-N1

Plasmid

#54645

Purpose

Localization: N1 Cloning Vector, Excitation: 598, Emission: 625

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mRaspberry

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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mOrange-C1

Plasmid

#54680

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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mPlum-N1

Plasmid

#54629

Purpose

Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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mApple-C1

Plasmid

#54631

Purpose

Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pME-QF

Plasmid

#61375

Purpose

Middle Gateway Entry Vector Containing QF

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QF

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSM019

Plasmid

#128291

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sfyfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-8xPP7

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGPI-SceI

Plasmid

#32060

Purpose

Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Type

Empty backbone

Use

Mutagenesis plasmid

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFBDM

Plasmid

#110738

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pQUAST-GAL80

Plasmid

#46137

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL80 (GAL80 Budding Yeast)

Expression

Insect

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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mPlum-C1

Plasmid

#54839

Purpose

Localization: C1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI

Plasmid

#44182

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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