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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,764 results for: plat

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Showing: 4681 - 4700 of 8764 results

  1. TFORF0274

    Plasmid
    #142581
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF3 (TCF3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142581-plasmid-map-sequence-id-385951

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  2. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Publication
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23106
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

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  13. pLCHKO_Ptbp_ex8_3

    Plasmid
    #155083
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155083-plasmid-map-sequence-id-308532

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  14. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid
    #121806
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121806-plasmid-map-sequence-id-237315

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  15. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid
    #121812
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121812-plasmid-map-sequence-id-237358

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  17. FB002

    Plasmid
    #119676
    Purpose
    Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator tub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119676-plasmid-map-sequence-id-237306

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  18. FB008

    Plasmid
    #119706
    Purpose
    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119706-plasmid-map-sequence-id-237146

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Showing: 4681 - 4700 of 8764 results