We narrowed to 9,649 results for: virus

1. pAAV-EF1a-iFlpV

   Plasmid

   #140137

   Purpose

   can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iFlpV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-iDreV

   Plasmid

   #140136

   Purpose

   can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iDreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+HyPer7-NLS

   Plasmid

   #136468

   Purpose

   Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Triple nuclear localization signal of SV40 (simia…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLXSN p200 CUX1

   Plasmid

   #90470

   Purpose

   Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

   Depositor

   Alain Nepveu

   Article

   Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Retroviral

   Tags

   Ha and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney murine leukemia virus long terminal repeat

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE7-P2A-BSD

   Plasmid

   #224122

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-BSD
   

   Use

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PEmax-P2A-BSD

   Plasmid

   #174821

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-BSD
   

   Use

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207862

   Purpose

   AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV pmSyn1-EBFP-Cre

    Plasmid

    #51507

    Purpose

    Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV5

    Insert

    EBFP-Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pmSyn1

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

    Plasmid

    #120561

    Purpose

    Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid

    #182671

    Purpose

    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.

    Depositor

    Bas van Steensel

    Article

    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do

    Insert

    Lamin B1 (LMNB1 Human)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207859

    Purpose

    AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65455

    Purpose

    Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge