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pCfb13212

Plasmid

#219887

Purpose

The base plasmid of TUNEYALI for TF22

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13202

Plasmid

#219877

Purpose

The base plasmid of TUNEYALI forTF12

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13234

Plasmid

#219907

Purpose

The base plasmid of TUNEYALI for TF44

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13219

Plasmid

#219894

Purpose

The base plasmid of TUNEYALI for TF29

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13248

Plasmid

#219919

Purpose

The base plasmid of TUNEYALI for TF58

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2

Plasmid

#204976

Purpose

Co-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tag

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A pMT2

Plasmid

#206110

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_6F→A

Plasmid

#194269

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

F276A, F283A, F289A, F367A, F397A, F401A

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_CR

Plasmid

#194263

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M322A, M323A, M336A, M337A, M339A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_IDR

Plasmid

#194264

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307A, M311A, M359A, M405A, M414A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→L_IDR

Plasmid

#194265

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307L, M311L, M359L, M405L, M414L mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→Y_IDR

Plasmid

#194266

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5A→M_IDR

Plasmid

#194267

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

A297M, A367M, A381M, A388M, A391M mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-luciferase

Plasmid

#198590

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

luciferase

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pJR035

Plasmid

#198821

Purpose

For tissue specific RNA-seq

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJR015

Plasmid

#198812

Purpose

Epidermal growth factor

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::Lin-3c-SL2-GFP::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJR026

Plasmid

#198824

Purpose

Lysosomal associated membrane protein

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::lmp1-venus::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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