We narrowed to 59,215 results for: %s

1. pGWB455

   Plasmid

   #74837

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PE2-BSD

   Plasmid

   #161514

   Purpose

   Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

   Insert

   PE2-P2A-BSD
   

   Use

   Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α core promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpRY-YE1-BE4max

   Plasmid

   #198554

   Purpose

   Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB555

   Plasmid

   #74885

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWB405

   Plasmid

   #74799

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB441

   Plasmid

   #74827

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGWB540

   Plasmid

   #74874

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB442

    Plasmid

    #74828

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p6622 MSCV-P C HA GLTSCR

    Plasmid

    #34902

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    GLTSCR1 (BICRA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-nls-sfGFP

    Plasmid

    #132973

    Purpose

    full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nuclear localized super folder GFP
    

    Use

    Gateway vector

    Tags

    6x myc and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB515

    Plasmid

    #74857

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HR180-LGR5-CreER

    Plasmid

    #129093

    Purpose

    KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    CreERT2 and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB404

    Plasmid

    #74798

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpCas9-NRCH-YE1-BE4max

    Plasmid

    #198549

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGMCtKq28-TSC10M1-sspB

    Plasmid

    #49526

    Purpose

    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hsp68-LacZ-Gateway

    Plasmid

    #37843

    Depositor

    Nadav Ahituv

    Article

    Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Mouse Hsp68 minimal promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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