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pBiT3.1-N_CORO1B:1-489

Plasmid

#211196

Purpose

Mammalian expression of full-length human CORO1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1B:M1-A489 (CORO1B Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_AAMP:1-434

Plasmid

#211192

Purpose

Mammalian expression of full-length human AAMP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

AAMP:M1-R434 (AAMP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR38:1-314

Plasmid

#211329

Purpose

Mammalian expression of full-length human WDR38. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR38:M1-T314 (WDR38 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_KCTD3:1-815

Plasmid

#211286

Purpose

Mammalian expression of full-length human KCTD3. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

KCTD3:M1-L815 (KCTD3 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR53:1-358

Plasmid

#211256

Purpose

Mammalian expression of full-length human WDR53. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR53:M1-F358 (WDR53 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_BOP1:1-746

Plasmid

#211004

Purpose

Mammalian expression of full-length human BOP1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-N_WDR91:1-747

Plasmid

#210998

Purpose

Mammalian expression of full-length human WDR91. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR91:1-747

Plasmid

#210999

Purpose

Mammalian expression of full-length human WDR91. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/36]

Plasmid

#199422

Purpose

Mammalian expression of Ankyrin-G scFv. Derived from hybridoma N106/36.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingAnkyrin-G (Human) (ANK3 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2A-ΔC-S227A

Plasmid

#200556

Purpose

Mammalian expression of secreted mouse CES2A S227A mutant

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Publication

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KAP3B

Plasmid

#193939

Purpose

Mammalian expression of KAP 3B

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

KAP 3B (KIFAP3 Human)

Tags

FRB

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP10

Plasmid

#180343

Purpose

mammalian expression of human SEPT9_i1 fused to GFP10

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfCsf1a

Plasmid

#168103

Purpose

Express soluble His-tagged zebrafish Csf1a in insect cells

Depositor

Petr Bartunek

Publication

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Csf1a (csf1a Zebrafish)

Tags

6x His

Expression

Insect

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

Plasmid

#182354

Purpose

Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

Plasmid

#182356

Purpose

Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_AURKB_Pkinase

Plasmid

#110025

Purpose

Protein expression and purification of AURKB_Pkinase

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AURKB_Pkinase (AURKB Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA-H1.4CTD

Plasmid

#157802

Purpose

Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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