We narrowed to 16,622 results for: puromycin

1. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MASTL B4.4 gRNA

   Plasmid

   #90755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MASTL

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MASTL (Guide Designation B4.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTet-SNAP-Centrin3

   Plasmid

   #136806

   Purpose

   Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Centrin3
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ki67 (LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183743

   Purpose

   Expresses Ki67 (LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   LR domain of Ki67

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIP-GFP-STX17ΔNTD

   Plasmid

   #89941

   Purpose

   Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 lacking N-terminal domain (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   deleted amino acids 1-161

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. IRAK1-UTR

   Plasmid

   #15095

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   IRAK1 3'UTR (IRAK1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-CMV-NGLY1-Puro

   Plasmid

   #181916

   Purpose

   Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   N-glycanase 1 (NGLY1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroSuper-shSrc

   Plasmid

   #26982

   Depositor

   Joan Massague

   Article

   Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

   Insert

   shRNA Src (SRC Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAIP

    Plasmid

    #74171

    Purpose

    Lentiviral vector for expression of heterologous genes

    Depositor

    Jeremy Luban

    Article

    Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

    Plasmid

    #100160

    Purpose

    Expresses PRKAR2B

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    PRKAR2B (PRKAR2B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV/TO V5-LUC Puro (w549-1)

    Plasmid

    #19785

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-NFLAG TRF1 deltaA deltaM

    Plasmid

    #16065

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    Deleted acidic domain (aa1-66) and myb domain (aa…

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pMXs-IP-EGFP-ULK1(K46N)

    Plasmid

    #38197

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Asparagine

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

17. TRAF6-UTR

    Plasmid

    #15093

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    TRAF6 3'UTR (TRAF6 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

18. KIF20A D5.2 gRNA

    Plasmid

    #90718

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF20A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF20A (Guide Designation D5.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-mCherry-SPASTIN

    Plasmid

    #180647

    Purpose

    Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, has siRNA resistance to GAACAGUGUG…

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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