We narrowed to 13,494 results for: Mpl;

1. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   C. neoformans U6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVenusNfN

   Plasmid

   #128564

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVenusCfC

   Plasmid

   #128567

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVenusCfN

   Plasmid

   #128566

   Purpose

   Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

   Depositor

   Oliver Vugrek

   Article

   Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

   Insert

   Venus
   

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc EDC4 pCMVSport6

   Plasmid

   #86855

   Purpose

   mammalian expression of EDC4

   Depositor

   Sarah Slavoff

   Article

   D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

   Insert

   EDC4 (EDC4 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPUR-mU6-sgRNA-2XPP7

   Plasmid

   #194504

   Purpose

   Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT/FLAG/HA-DEST QKI

   Plasmid

   #19891

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159872

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid

    #159873

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1_Cas9-Ck4

    Plasmid

    #136135

    Purpose

    L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:Cas9_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIZ-Flag6His-BmAgo3

    Plasmid

    #50559

    Purpose

    Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    BmAgo3
    

    Tags

    6xHIS and Flag

    Expression

    Insect

    Promoter

    OpIE2

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcdna nesprin TS

    Plasmid

    #68127

    Purpose

    nesprin tension sensor

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    mouse nesprin mini 2G (Syne2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN mCherry-OPTN(D474N)

    Plasmid

    #119683

    Purpose

    For mammalian expression of UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D474N

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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