We narrowed to 4,802 results for: pcr
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Plasmid#213205PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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HA-MBP-TEVpCS-ADGRF2 CTF-FLAG
Plasmid#217695PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-429Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS FP>IA-HA-GFP
Plasmid#199688PurposeExpression of LYCHOS FP>IA mutant in insect cellsDepositorInsertLYCHOS (GPR155 Human)
Tags10xHis, 6xHis, EGFP, HA, and HRV 3CExpressionInsectMutationF43 and P44 mutated to I and A respectivelyAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC2-lifeact-mCherry
Plasmid#129558PurposepClimDCLifeAct:mCh actin filaments markerDepositorInsertlifeAct fused to mCherry
ExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHC1-mIAA17 Hygro
Plasmid#140544PurposeDHC1 tagging with mIAA7DepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
IgH P+ VA20
Plasmid#127249PurposeIgH V-regionDepositorInsertPromoter+V-region of IgH of A20 B cell line
UseOtherPromoterIgHAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR137
Plasmid#226809PurposeGateway entry vector for GPR137DepositorInsertGPR137 (GPR137 Human)
UseGateway CloningTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_001164351Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
OPRL1-DuET
Plasmid#213360PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTF-FLAG
Plasmid#217694PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-566Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED 4CA-FLAG
Plasmid#199681PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-FlagDepositorInsertLeucine zipper LYCHOS LED 4CA (GPR155 Human)
Tags6xHis and FlagExpressionBacterialMutationC595.604.629.638 of LYCHOS mutated to APromoterT7 promoterAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED Y551A-FLAG
Plasmid#199680PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-FlagDepositorInsertLeucine zipper LYCHOS LED Y551A (GPR155 Human)
Tags6xHis and FlagExpressionBacterialMutationY551 of LYCHOS mutated to APromoterT7 promoterAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC2-H2B2-Venus
Plasmid#129556PurposepClimDC-H2B:mV nuclear markerDepositorInsertHistone 2B fused to venus
ExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA9
Plasmid#158487PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codonDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA1
Plasmid#158479PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codonDepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-2
Plasmid#109006PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK816
Plasmid#257341PurposeEncodes the 3’ end of S. rosetta PTSG_03655 CDS (without STOP codon) fused to a linker and followed by mStayGold CDS and the 3’ UTR region of S. rosetta PTSG_03655.DepositorInsertPTSG_03655_3'CDS-recovery-sequence-linker-mStayGold-PTSG_03655-3’UTR
TagsmStayGoldExpressionBacterialMutationSynonymous codon mutations made to PTSG_03655 3…Available SinceJune 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA HCAR3
Plasmid#226807PurposeGateway entry vector for 2xHA HCAR3DepositorInsertHCAR3 (HCAR3 Human)
UseGateway CloningTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_006009.2Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
DHC1-mIAA7 Neo
Plasmid#140543PurposeDHC1 tagging with mIAA7DepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA3
Plasmid#158481PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codonDepositorAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA2
Plasmid#158480PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codonDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only