We narrowed to 18,827 results for: Mut

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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placZα-tdMCP

Plasmid

#191132

Purpose

Expression of tdMCP from a lacZα inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

dMCP_1

Use

Synthetic Biology

Promoter

lac-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Expression

Insect

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pGH183_MS2-AIDΔDead-Hygro

Plasmid

#85414

Purpose

lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔDead and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CP1028-CBEmax

Plasmid

#119802

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1028) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Puro.let-7gsm.Cre

Plasmid

#17410

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g mutant

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

mutated version of let-7g

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSV2 neuT

Plasmid

#10919

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuT (Erbb2 Rat)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
epi-ABEmax

Plasmid

#135974

Purpose

Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

Plasmid

#202023

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)

Expression

Bacterial

Mutation

GvpA T6A-I48V

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSV2 neuN

Plasmid

#10917

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuN (Erbb2 Rat)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pSC12

Plasmid

#104786

Purpose

pNB184-Cas9 entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pXEE401BG

Plasmid

#91717

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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VRER-ABEmax

Plasmid

#119812

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSC10

Plasmid

#104784

Purpose

pAH595-gRNA 2xplex entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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