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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#244109

Purpose

Cytosolic expression of non-responsive circularly permuted Super Folder GFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

cpSFGFP

Use

AAV

Tags

HaloTag

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2

Plasmid

#244111

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mem).iGlucoSnFR2

Plasmid

#244077

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2

Plasmid

#244081

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV CB6 FFLuc

Plasmid

#35655

Depositor

Phillip Zamore

Publication

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-dTom

Plasmid

#178716

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-GFP

Plasmid

#178710

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GFP

Plasmid

#178711

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mGreenLantern_FRT

Plasmid

#258655

Purpose

FlpO dependent mGreenLantern expression plasmid

Depositor

Franck Polleux

Insert

mGreenLantern

Use

AAV

Promoter

CAG

Available since

Aug. 4, 2026

Availability

Academic Institutions and Nonprofits only

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AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905)

Plasmid

#199774

Purpose

enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

wtGFP plus mutations F46L F64L S65G S72A M153T V1…

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183536

Purpose

Optogenetics

Depositor

Karl Deisseroth

Publication

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

None

Promoter

CaMKIIa

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

Plasmid

#176281

Purpose

Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Publication

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBacMUT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

E191K

Promoter

human Synapsin I

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Soma-jGCaMP8f

Plasmid

#182943

Purpose

Cell body-targeted jGCaMP8f, under synapsin promoter.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-jGCaMP8f

Use

AAV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shCrh

Plasmid

#132715

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

Depositor

Robert Messing

Publication

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shCrh (Crh Rat)

Use

AAV

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Nrxn-V5-TurboID-NES

Plasmid

#250172

Purpose

Membrane-TurboID variant for neuron expression

Depositor

Christina Kim

Insert

Nrxn (NRXN1 Human)

Use

AAV

Tags

HA (internal) and V5-TurboID-NES

Expression

Mammalian

Promoter

hSyn

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#219782

Purpose

CaST-IRES for HEK cell expression

Depositor

Christina Kim

Publication

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2

Plasmid

#171846

Purpose

Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

Depositor

Richard Huganir

Publication

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

Plasmid

#113156

Purpose

An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat Rosa26

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGIG-Malat_Barcode_0

Plasmid

#250803

Purpose

Expresses a molecular barcode sequence attached to the nuclear localization sequence of the lncRNA Malat for enhanced detection in single nucleus sequencing libraries

Depositor

Murray Blackmore

Insert

Malat_Barcode_0

Use

AAV

Expression

Mammalian

Promoter

CAG or CAGIG

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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