We narrowed to 568 results for: BR1

1. pLV_PGK_3xHA-LTB4R

   Plasmid

   #208091

   Purpose

   Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 SEC66-VAC8-GFP

   Plasmid

   #166840

   Purpose

   Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Use

   Tags

   GFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LTB4R-Tango

   Plasmid

   #66423

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV_PGK_3xHA-LTB4R(T308A)

   Plasmid

   #208092

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(T308A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T308A

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_PGK_3xHA-LTB4R(S310A)

   Plasmid

   #208093

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(S310A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   S310A

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_PGK_3xHA-LTB4R(T308A,S310A)

   Plasmid

   #208094

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(T308A,S310A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T308A, S310A

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_003-sgUBR4

   Plasmid

   #202442

   Purpose

   Expression of a guide RNA targeting UBR4

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   UBR4 gRNA (UBR4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROS_phleo-Tps1/Gsy2

   Plasmid

   #196612

   Purpose

   Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-OptoTGFBRs

   Plasmid

   #118942

   Purpose

   Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-HA-Nr4a1

    Plasmid

    #160941

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-BRPF1

    Plasmid

    #65382

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRPF1 (BRPF1 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDONR221_SLC7A11

    Plasmid

    #132244

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNCX2-OptoTGFBRs

    Plasmid

    #118965

    Purpose

    Retroviral expression OptoTGFBRs for transient or stable constitutive expression

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
    

    Use

    Retroviral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPR v2-Nr4a1sgRNA1

    Plasmid

    #160945

    Purpose

    Guide RNA 1 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 1 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20 2xHA LTB4R

    Plasmid

    #226810

    Purpose

    Tet-inducible lentiviral vector for 2xHA LTB4R expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_858043.1

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A 2xHA LTB4R

    Plasmid

    #226806

    Purpose

    Gateway entry vector for 2xHA LTB4R

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_858043.1

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LTB4R-DuET

    Plasmid

    #213333

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF3462

    Plasmid

    #144938

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BRPF1 (BRPF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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