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Plasmid#170353PurposemTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H127
Plasmid#170343Purpose6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H151
Plasmid#170346Purpose6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterSP6Available sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9
Plasmid#154929PurposeaTc inducible HypaCas9DepositorInsertHypaCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698APromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_2pabPAC(Y72L)
Plasmid#158970PurposeA negative control for AAV-mediated 2P-activatable bPACDepositorInsert2pabPACY72L
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglCDEF
Plasmid#134677PurposeExpresses TglC, TglD, TglE, TglF in E. ColiDepositorInsertsTglC
TglD
TglE
TglF
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglB
Plasmid#134676PurposeExpresses His6-TglBin E. ColiDepositorInsertTglB
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-ammA(∆50-56)
Plasmid#134684PurposeExpresses His6-AmmA(∆50-56) in E. ColiDepositorInsertammA(del50-56)
UseTagsHis6ExpressionBacterialMutationdeletion of amino acids 50-56PromoterT7Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-ammA
Plasmid#134682PurposeExpresses His6-AmmA in E. ColiDepositorInsertammA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-HypaCAS9-pA
Plasmid#131467PurposeExpresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized HypaCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-cfp
Plasmid#127944PurposeCFP fluorescent reporter for AcrABDepositorInsertPacrAB-cfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PaccD (-82 to +23)
Plasmid#123464PurposeMoChlo kitDepositorInsertPaccD (-82 to +23)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-QKI__PACRG
Plasmid#116835PurposeLentiviral expression of QKI__PACRG fusionDepositorInsertQKI__PACRG
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CDK6__HEPACAM2
Plasmid#116812PurposeLentiviral expression of CDK6__HEPACAM2 fusionDepositorInsertCDK6__HEPACAM2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI_MafB_ISH
Plasmid#87670PurposeIn situ probe for mouse MafbDepositorInsertMafb (Mafb Mouse)
UseTagsExpressionBacterialMutationPromoterT7/T3Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYCduet-mxLOX2-EH
Plasmid#104979PurposeBiosynthesis of oxylipins by microbial enzymesDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Dhr1
Plasmid#100003PurposeExpress yeast Dhr1 with Y2H activation domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Mrt4
Plasmid#99998PurposeExpress yeast Mrt4 with Y2H activation domain fusionDepositorInsertMrt4 (MRT4 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only